Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml
TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml.
Enzo Life Sciences IHC PBS tablets (100ea)
Formulation: Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer. Long Term Storage: Ambient. Handling: Protect from moisture. Applications: IHC .
Medchemexpress LLC PIPES dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 10 G
PIPES dipotassium is a zwitterionic buffering agent used in biochemical and biological assays to maintain a stable, near-neutral pH. The dipotassium salt provides good aqueous solubility and is compatible with many enzymatic and cell-based applications.
- Maintains pH in the 6.1-7.5 buffering range.
- Zwitterionic structure minimizes metal binding and enzymatic interference.
- High purity (98.0%).
- White to off-white solid, water soluble.
- Available in 10 G and larger lab-scale pack sizes.
- Store at room temperature; keep under nitrogen and away from moisture; in solution store at -20°C to -80°C for long-term storage.
- Supplied with SDS and COA documentation.
Boston Bioproducts Inc 50 mM Sodium Phosphate, 2 mM EDTA (pH 7.0) - 2x1L
Bioprocess Grade - 50 mM Sodium Phosphate 2 mM EDTA (pH 7 0) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.2) - 50ml
Sodium Phosphate Buffer (1 M pH 7 2) - 50ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.5) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 7 5) - 2x1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra, for molecular biology, 99.5 (T), 7365-45-9, MFCD00006158
HEPES BioUltra, for molecular biology, 99.5 (T)
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 1kg
BIS-TRIS | Purity: ≥98.0% (titration) | MW:209.24 | 6976-37-0 | MFCD00002853 | 1kg