Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 2306265-70-1 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159806.JPG-250.jpg)
eMolecules 2306265-70-1 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 1g
Medchem Express | Ginsenoside F1 | 5mg | 446275202 | HY-N0598 | 53963-43-2 | MFCD06410947 | 638.883 | C36H62O9
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Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
CHEMSCIENCE Brass "A" for Sn, Pb, Cu, Zn
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
Boston Bioproducts Inc Tris-Base (2 M, pH > 10.0) - 250ml
Tris-base is a widely used biological buffer in various research applications in biochemistry, molecular biology, and cell biology. The buffering capabilities of Tris-base are effective in the pH range of 7.0-9.0, making it suited for various biological applications. This buffer is widely used in protocols for DNA and RNA extraction, in situ hybridization, protein extraction from cells, in agarose gel electrophoresis, polyacrylamide gel electrophoresis and as a wash buffer in Western blotting.
Boston Bioproducts Inc 10mM Tris-HCl pH 7.5 1L
Tris-HCl Buffer (10 mM pH 7 5) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (10X) - 4L
Tris-Acetate-EDTA Buffer TAE (10X) - 4L (For Research Use and Further Manufacturing Only)
Bioworld TT - SDS Running Buffer (Cathode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.3. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
National Diagnostics SEQUAGEL BUFFER 200 ML
SequaGel Buffer, System consists of SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer for casting 19:1 denaturing DNA gels of a varying percentage, 200 ML
Teknova 50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.