Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 20L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Teknova HBS-EP Buffer (10mM Hepes, 150mM NaCl, 3mM EDTA, 0.005% Tween-20) 200mL, Sterile. 10 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 0.5M Tris, 1.5M Sodium Chloride, 0.5% Tween-20, pH 7.7. 500mL, Sterile.
0.5M Tris,1.5M Sodium Chloride,0.5% Tween-20
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Teknova 1M Tris-HCl, pH 7.8 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 2000mL Cube
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ TBE 10X buffer (Tris-borate EDTA), 500mL
Alkali Scientific™ CellPro™ TBE (Tris-borate EDTA) solution offers a sterile and filtered formulation of this essential buffer for molecular biology and electrophoresis applications. With its meticulous preparation, this solution ensures purity and consistency, meeting the rigorous demands of scientific research and experimentation. The sterile nature of the solution minimizes the risk of contamination, making it ideal for various molecular biology techniques, including DNA and RNA agarose gel electrophoresis. Additionally, the filtered formulation guarantees clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used for nucleic acid separation, DNA fragment analysis, or other molecular biology procedures, CellPro™ TBE solution delivers consistent performance and reproducible results, supporting the success of laboratory workflows.
Research Products International Corp TAPS, 100 G
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Research Products International Corp TG 10X Solution, 1 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
Research Products International Corp RPI TRIS-CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
This product is shipped with one 500 ml Anode bottle and one 500 ml Cathode bottle
10X Anode Formulation:
TRIS: 0.6 M
CAPS: 0.4M
10X Cathode Formulation:
TRIS: 0.6 M
CAPS: 0.4 M
SDS: 1%
Research Products International Corp Stacking Buffer 4X Solution, 500 ML
4X concentrated buffer solution for preparation of Laemmli SDS-PAGE resolving gels.
Research Products International Corp Tricine-SDS Sample Buffer 2X Solution, 25 ML
2X Formulation:
SDS: 8%
Glycerol: 20%
Coomassie Blue: 0.01%
Phenol Red: 0.01%
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-50.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 50 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.